X-rays and Protein Crystal Samples

Lessening X-ray damage is healthy for protein discovery data too

Image credit: Wayne Anderson, Northwestern University.

Protein crystal samples are placed on a small metal tip so x-rays from the adjacent beam pipe can pass through them and diffract off the atoms inside the crystal. Using computational methods, scientists interpret the scattered light patterns recorded by detectors to create an image of how the atoms are located inside the crystal.

ANL Article

Karolina Michalska, Kemin Tan, Hui Li, Catherine Hatzos-Skintges, Jessica Bearden, Gyorgy Babnigg, and Andrzej Joachimiak. “GH1-family 6-P-β-glucosidases from human microbiome lactic acid bacteria”Acta Cryst. (2013). D69, 451-463. DOI: 10.1107/s0907444912049608

Funding Acknowledgements: Midwest Center for Structural Genomics (MCSG) and Structural Biology Center (SBC) at Argonne National Laboratory (ANL): support. Funding in part: grant from National Institutes of Health (NIH; GM094585) and by the Office of Biological and Environmental Research (OBER), U.S. Department of Energy (DOE) Office of Science, under Contract DE-AC02-06CH11357. Submitted manuscript created by University of Chicago Argonne, LLC, operator of ANL, a DOE Office of Science Laboratory, is operated under Contract No. DE-AC02-06CH11357.