Chemical and Elemental Information

A widely used method of synchrotron infrared hyperspectral imaging is synchrotron-based Fourier-transform infrared imaging (sFTIR), or sFTIR spectromicroscopy. This technique correlates infrared maps with visible microscopy images to map the distributions of molecular compositions of interest within biological samples and to reveal sample morphology and structure.

• Synchrotron Infrared Hyperspectral Imaging technique details
• Resources offering this technique:
  • Berkeley Synchrotron Infrared Structural Biology at ALS

Synchrotron-based X-ray spectroscopy techniques provide a powerful and synergistic toolkit to explore metal interactions within biological and biogeochemical systems and with the environment.

• X-Ray Absorption and Emission Spectroscopy technique details
• BER resources offering this technique:
  • eBERlight at APS
  • Structural Molecular Biology at SSRL

X-ray fluorescence imaging (XRF), or x-ray spectromicroscopy, maps the distributions of elements and chemical species of interest within biological samples. Synchrotron XRF (SXRF) can provide detailed images of element speciation to a resolution of 0.5 µm per pixel, a sensitivity beyond desktop XRF, electron microprobe, or other elemental imaging techniques.

• X-ray Fluorescence Imaging technique details
• Resources offering this technique:
  • Beamline 10.3.2 — Advanced Light Source
  • Beamline 13-IDE — Advanced Photon Source
  • Beamline 4-BM — National Synchrotron Light Source-II
  • Beamlines 2-3, 10-2, 14-3 — Stanford Synchrotron Radiation Lightsource
  • eBERlight at APS