BER Structural Biology and Imaging Resources
Synchrotron, Neutron, and Cryo-EM
U.S. Department of Energy | Biological and Environmental Research Program

Chemical and Elemental Information

The following are imaging and characterization techniques that can be used to study chemical and elemental information.

Synchrotron Infrared Hyperspectral Imaging

A widely used method of synchrotron infrared hyperspectral imaging is synchrotron-based Fourier-transform infrared imaging (sFTIR), or sFTIR spectromicroscopy. This technique correlates infrared maps with visible microscopy images to map the distributions of molecular compositions of interest within biological samples and to reveal sample morphology and structure.

X-Ray Absorption and Emission Spectroscopy

Synchrotron-based X-ray spectroscopy techniques provide a powerful and synergistic toolkit to explore metal interactions within biological and biogeochemical systems and with the environment.



X-Ray Fluorescence Imaging

X-ray fluorescence imaging (XRF), or x-ray spectromicroscopy, maps the distributions of elements and chemical species of interest within biological samples. Synchrotron XRF (SXRF) can provide detailed images of element speciation to a resolution of 0.5 µm per pixel, a sensitivity beyond desktop XRF, electron microprobe, or other elemental imaging techniques.